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. 2023 Feb 15;14:1069348. doi: 10.3389/fphar.2023.1069348

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Copyright © 2023 Li, Ma, Wang, Zhang, Liu, Liu, Huang, Zhu, Zhang, Yin, Xu, Ding, Liu, Shi and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction between ARAP1 and CIN85 (A) Forty-eight hours after transfection of ARAP1 shRNA in the NG group and HG group, the protein expression of CIN85 was examined by Western blot analysis. (B) IHC detection of CIN85 in kidney tissues from db/m, db/db, db/db + AAV-shNC and db/db + AAV-shARAP1 mice (×400). Bar = 50 μm. Arrows indicate positive staining. (C) The effect of AAV2/9-shARAP1 on the protein expression of CIN85 in the isolated glomeruli lysates of mouse kidney tissues was examined by Western blot analysis. (D,E) The physical interaction between ARAP1 and CIN85 in human glomerular mesangial cells and mouse glomeruli was detected by Co-IP analysis. IgG was used as a negative control. In all panels, the data are representative of at least three independent experiments. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, NS, not significant.