Table 6.
Primer names and sequences, target size and PCR conditions for enterotoxin genes detection58.
| Primers | Target size (bp) | Sequences (5′–3′) | Reaction conditions |
|---|---|---|---|
|
hblA F hblA R |
1154 |
AAGCAATGGAATACAATGGG AGAATCTAAATCATGCCACTGC |
94 °C,2 min/(94 °C,60 s*56 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
|
hblC F hblC R |
740 |
GATACCAATGTGGCAACTGC TTGAGACTGCTCGCTAGTTG |
94 °C,2 min/(94 °C,60 s*58 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
|
hblD F hblD R |
829 |
ACCGGTAACACTATTCATGC GAGTCCATATGCTTAGATGC |
94 °C,2 min/(94 °C,60 s*58 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
|
nheA F nheA R |
499 |
TACGCTAAGGAGGGGCA GTTTTTATTGCTTCATCGGCT |
94 °C,2 min /(94 °C,60 s*56 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
|
nheB F nheB R |
769 |
CTATCAGCACTTATGGCAG ACTCCTAGCGGTGTTCC |
94 °C,2 min/(94 °C,60 s*54 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |
|
nheC F nheC R |
581 |
CGGTAGTGATTGCTGGG CAGCATTCGTACTTGCCAA |
94 °C,2 min/(94 °C,60 s*58 °C,60 s*72 °C,120 s)35cycles /72 °C,5 min |