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. 2023 Feb 28;14:947. doi: 10.1038/s41467-023-36496-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a WGA aggregates and induces endocytosis of glycosylated proteins in cells. After surface biotinylation, SkBr3 cells ± CytochalasinD (CytoD) were incubated with WGA for 10 min, and remaining surface biotin was removed. Samples were harvested after processing, as described in the “Methods” section, assayed by immunoblot for receptor tyrosine-protein kinase erbB-2 (HER2), epidermal growth factor receptor (EGFR), sodium/potassium-transporting ATPase Alpha1 (Na/K-ATPase) and transferrin-receptor 1 (TfR). Numbers indicate relative levels of uptake normalised to the control sample. b WGA uptake is mediated by both clathrin/dynamin-dependent and actin-dependent endocytic pathways. Cells transfected with dominant-negative DynaminS45N N-terminally GFP-tagged expression construct were incubated ± CytochalasinD (CytoD) with dylight650-labelled WGA and analysed by confocal microscopy. Scale bars: 10 µm. Source data are provided as a Source Data file.