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. 2023 Feb 28;14:1143. doi: 10.1038/s41467-023-36693-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A In red are indicated CIP2A(1-560) residues identified in inter-molecular cross-links to B56α using DSS and DMTMM cross-linkers. CIP2A 1-560 homodimer (PDB: 5UFL) monomers are shown either as ribbon or space filling models. In the space filling model 1-330 amino acids are in cyan including in purple the CIP2A head domain identified in this study. The 331-560 region including the dimerization domain is shown as turquoise. Due to high molecular flexibility of the N-terminal region of CIP2A, positions of residues K21 and E23 (indicated with*) had to be approximated using A24 position and K40 using position of R43. B Distribution of B56α (PDB: 6NTS) residues cross-linked to CIP2A by using DSS and DMTMM chemistries. Cross-linked amino acids are in red and roughly distributed to two distinct regions on B56α shown in two different B56α orientations. Insert shows the overall structural organization of PP2A-B56α trimer consisting of PP2A-A, PP2Ac, and B56α. C Overlap of the B56α cross-links identified for two different CIP2A fragments, 1-330 and 1-560. D Overlap of the CIP2A cross-links on B56α using CIP2A fragments, 1-330 and 1-560. D, E The indicated cross-link sites are combined from both the DSS and DMTMM experiments. E Zoom-into the CIP2A´s head domain, shown for one CIP2A monomer. CIP2A sites found in cross-links, with DSS and DMTMM, to B56α are in red, and the head domain (1-43 aa) is coloured in purple. Residue annotation is the same as in panel (A).