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. Author manuscript; available in PMC: 2024 Feb 16.
Published in final edited form as: Cell. 2023 Feb 6;186(4):864–876.e21. doi: 10.1016/j.cell.2022.12.041

Figure 5. CBASS escape phages have mutations in the major capsid gene.

Figure 5.

(A) Plaque assays were performed with the indicated control/WT and escape phages spotted in 10-fold serial dilutions on lawns of bacteria expressing CBASS+ (left) or lacking CBASS- (right); clearings represent phage replication. (B) Schematic of major capsid genes with corresponding missense mutations and associated CBASS Escape (ESC) phages. (C) Schematic of in vivo homologous recombination of parental phages with homology-directed repair (HDR) template 1 (encoding I121S or I121T capsid mutations) or template 2 (S330P capsid mutation) and resultant engineered/recombinant phages. (D) Plaque assays with recombinant phages possessing major capsid mutations, or WT capsid controls, spotted on lawns of Pa011 WT or ΔCBASS.