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SDS-PAGE analysis on a 4 to 20% polyacrylamide gradient gel under reducing conditions. Eluted and pooled fractions from HSA-affinity-purified cell wall surface proteins from recombinant S. carnosus cells as follows: pSPPmABPXM transformed (lane 1), Sc:mZIgA (lane 2), Sc:dZIgA (lane 3), Sc:mZIgE (lane 4), and Sc:dZIgE (lane 5). Lane M, marker proteins with molecular masses (in kilodaltons).
