Fig. 6.

Deficiency of NCOA3-p300-NF-κB members inhibited breast cancer cell proliferation, invasion, and in vivo tumor growth. A Cell viability. The Control-KD1, p65-KD1, p50-KD1, p300-KD1, and NCOA3-KD1 cells were used for the MTT assay after treatment with PBS or 10 nM E2 in PBS at different time points (0, 1, 2, 3, 4, and 5 days). Three independent replicates (n = 3 for each replicate) were performed, and the results represent the means of three replicates ± SD. B Colony numbers. Cells in (A) were used for the colony formation assay. C Invaded cell numbers. Cells in (A) were used for the cell invasion assay. For experiments in (B) and (C), three independent replicates (n = 3 for each replicate) were performed, and the results represent the means of three replicates ± SD. D Tumor volumes. Cells in (A) were injected into female nude mice (n = 10 for each cell line), followed by implantation or no implantation of a 0.18 mg E2 pellet. Tumor volumes were measured at 5-day intervals for 40 days. Three independent replicates (n = 10 for each replicate) were performed, and the results represent means of three replicates ± SD. Significant differences in this figure were determined by one-way ANOVA, followed by Tukey's post hoc test. * P < 0.05 and ** P < 0.01