FIGURE 1.

Leukocyte intravascular adhesion contributes to vascular leakage during EAE. In vivo two photon laser scanning microscopy was performed on exposed spinal cord of an EAE mouse at disease peak. Pial vessels were visualized through the intravenous injection of 70,000 kDa molecular weight FITC-dextran. White arrows indicate leakage of FITC-dextran into the extravascular space at the point where circulating leukocytes (detected by the imaging system as black dots) perform intravascular adhesion. Representative sequential images at T0 (A), after 40 s (B), and 80 s (C). Adapted and modified from Dusi et al., 2019, (supplementary videos 1 and 2).