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SDS-PAGE of the purified extracellular (Ex) and periplasmic (Pe) isoamylase produced by E. coli JM109(pIF13). The purified enzymes were subjected to SDS-PAGE and detected with Coomassie Brilliant Blue R-250 (A) and by Western blotting (B). Mr markers: phosphorylase a (97,000), bovine serum albumin (66,000), glyceraldehyde 3-phosphate dehydrogenase (36,000), and soybean trypsin inhibitor (20,100).
