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. 2022 Dec 13;13(3):598–615. doi: 10.1158/2159-8290.CD-22-0968

Figure 2.

Figure 2. NVL-520 inhibits the viability of ROS1-driven cancer cell lines. A–C, Heat map showing the activity of TKIs in 3-day cell viability assays against wild-type ROS1 (A), ROS1 G2032R (B), or ROS1 with other resistance mutations (C). Numerical IC50 values (nmol/L) are provided. Each row represents a distinct cell line, with identity of the ROS1 fusion partner and the resistance mutation (if applicable) indicated. “IC50 <” and “IC50 >” are treated as “IC50 = ” for heat map coloring. Grayed-out entries indicate data not available (“NA”). All data represent geometric mean IC50 or best-fit IC50 across n ≥ 2 repeat testing. Errors and repeats are provided in Supplementary Fig. S4A. Average potencies for wild-type ROS1 and for ROS1 G2032R are presented as a separate row and highlighted in blue text, representing the geometric mean of IC50s across cell lines. D–E, Plots of data in Fig. 2B (D) and Fig. 2C (E). For each TKI, individual dots represent the IC50 against distinct cell lines, and the horizontal bar represents average potency (geometric mean with geometric standard deviation).

NVL-520 inhibits the viability of ROS1-driven cancer cell lines. A–C, Heat map showing the activity of TKIs in 3-day cell viability assays against wild-type ROS1 (A), ROS1 G2032R (B), or ROS1 with other resistance mutations (C). Numerical IC50 values (nmol/L) are provided. Each row represents a distinct cell line, with the identity of the ROS1 fusion partner and the resistance mutation (if applicable) indicated. “IC50 <” and “IC50 >” are treated as “IC50 = ” for heat map coloring. Grayed-out entries indicate data not available (NA). All data represent geometric mean IC50 or best-fit IC50 across n ≥ 2 repeat testing. Errors and repeats are provided in Supplementary Fig. S4A. Average potencies for wild-type ROS1 and for ROS1 G2032R are presented as a separate row and highlighted in blue text, representing the geometric mean of IC50s across cell lines. D and E, Plots of data in Fig. 2B (D) and Fig. 2C (E). For each TKI, individual dots represent the IC50 against distinct cell lines, and the horizontal bar represents average potency (geometric mean with geometric standard deviation).