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. 2023 Jan 17;42(5):e110468. doi: 10.15252/embj.2021110468

Figure 2. GSDMD is processed upon IAP targeting even in the absence of caspase‐1 or NLRP3.

Figure 2

  1. BMDMs were seeded at a density of 4 × 105 cells per well, primed with 20 ng/ml of LPS for 3 h then treated, as indicated, with 500 nM of Cp. A or 711 for 20 h and cell lysates and supernatants were analysed by western blot. Ponceau staining depicts protein loading. Data representative of two independent experiments.
  2. BMDMs of the indicated genotypes were seeded at a density of 5 × 105 cells per well and primed with 100 ng/ml of LPS for 3 h then treated, as indicated, with Cp. A (1 μM), 5Z‐7 oxozeaenol (TAK1i, 250 nM) for 3 or 6 h or nigericin (10 μM) for 45 min. Cell lysates and supernatants were analysed by western blot. Ponceau staining depicts protein loading. Data representative of two independent experiments.

Source data are available online for this figure.