Figure 6. In the absence of the downstream apoptotic machinery, caspase‐8‐mediated cleavage and activation of GSDMD are required for cell death upon IAP inhibition.

• A
  iBMDMs of the indicated genotypes were seeded at a density of 2 × 10⁵ cells per well and were primed with 50 ng/ml LPS for 3 h then treated with Cp. A (2 μM) for 24 h. Cell viability was determined by PI staining and flow cytometry and measured as a proportion of PI‐negative (live) cells. Data represent the mean of three independent experiments (symbols). Error bars are the mean ± SD.
• B, C
  iBMDMs of the indicated genotypes seeded at a density of 2 × 10⁵ cells per well were treated with ABT‐737 (1 μM) and CHX (20 μg/ml) for 6 h, or LPS (50 ng/ml) and Cp. A (2 μM) and IDN‐6556 (10 μM) for 24 h, or with LPS (50 ng/ml) for 3 h followed by nigericin (10 μM) for 1.5 h. Cell viability was determined by PI staining and flow cytometry and measured as a proportion of PI‐negative (live) cells. Data represent the mean of three independent experiments (symbols). Error bars are the mean ± SD.
• D
  Schematic model for how intrinsic and extrinsic cell death signalling are executed in macrophages.

Source data are available online for this figure.