Figure 7. In the absence of apoptotic and pyroptotic caspases, IAP loss triggers caspase‐8‐mediated processing of IL‐1β and GSDMD to allow IL‐1β activation and release.

• A, B
  iBMDMs of the indicated genotypes were seeded at a density of 4 × 10⁵ cells per well and primed with either 50 ng/ml (A) or 100 ng/ml (B) of LPS for 3 h then treated with Cp. A (2 μM) for 24 h. Cell supernatants were analysed by ELISA for levels of IL‐1β and TNF, as indicated. Data represent the mean of two independent experiments, error bars are the mean ± SD.
• C
  iBMDMs of the indicated genotypes were seeded at a density of 4 × 10⁵ cells per well and primed with 100 ng/ml of LPS for 3 h then treated with Cp. A (2 μM) for 24 h. As control stimuli, WT iBMDMs were primed with LPS (100 ng/ml) for 3 h then treated with nigericin (10 μM) for 1.5 h or ABT‐737 (1 μM) and CHX (20 μg/ml) for 6 h. Total cell lysates and supernatants were analysed by western blot. Ponceau staining depicts protein loading. Data representative of four independent experiments.

Source data are available online for this figure.