TABLE 1.
Lactate, ethanol, and acetate productions from wild-type, heterolactic, and homolactic K. lactis strains during batch growth on 5% (wt/vol) glucose-yeast nitrogen base medium
| Strain | Concentration of (mM)a:
|
Yieldb | U of LDHc | ||
|---|---|---|---|---|---|
| Lactate | EtOH | Acetate | |||
| PM6-7A | 0.0 | 161.0 | 0.5 | 0.00 | 0.0 |
| PM6-7A(pEPL2) | 47.7 | 84.8 | 0.3 | 0.17 | 4–5 |
| PMI/C1 | 0.0 | 0.0 | 1.7 | 0.00 | 0.0 |
| PMI/C1(pEPL2) | 126.5 | 0.0 | 0.3 | 0.46 | 55–60 |
a
Highest values measured. Numbers did not vary by more than 10% between independent experiments.
b
Yield is expressed as millimoles of lactic acid produced liter−1 divided by millimoles of glucose consumed liter−1.
c
Specific activity of the heterologous LDH enzyme. Units are given per milligram of total cell protein.