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. 2023 Feb;193(2):201–212. doi: 10.1016/j.ajpath.2022.11.002

Figure 1.

Figure 1

Induced pluripotent stem cell–derived cortical neurons express neuronal markers. A: Immunofluorescence images of MAP2 (green) and DAPI (blue) staining of neurons 8 weeks post-differentiation (see also Supplemental Figure S2). B: Western blot analysis of lysate from induced pluripotent stem cell–derived cortical neurons (ic-neurons) for synaptic markers PSD-95 (Postsynaptic Density 95) and synaptophysin, with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) loading control. C: Quantification of Western blots in panel B, with protein levels expressed as a ratio to GAPDH, normalized to the mean of both control lines. Data are expressed as means ± SD. n = 3 independent differentiation experiments (C). PSD-95, analysis of variance, P = 0.04, F∗(DFn, DFd) 4.43 (3, 8), post hoc HFF1s versus PgATLX, P = 0.065. Synaptophysin (SYNAP), analysis of variance, P = 0.46, F∗(DFn, DFd) 0.96(3, 8) (C). Scale bars = 100 μm (A).