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. 2023 Feb;193(2):201–212. doi: 10.1016/j.ajpath.2022.11.002

Figure 2.

Figure 2

POLG mutant induced pluripotent stem cell–derived cortical neurons (ic-neurons) show increased cell death, decreased dendrite length, and decreased POLG expression. A: Representative immunofluorescence images of DAPI (blue in merged image) and propidium iodide (PI; red in merged image) staining of neurons 8 weeks post-differentiation. B: Quantification of percent PI-positive nuclei. C: Quantification of total dendrite length per neuron. D: Representative images of each line expressing green fluorescent protein to highlight individual neurons for neurite tracing. E: Determination of quantitative RT-PCR measurements of POLG normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). F: Western blot analysis of lysate from ic-neurons using antibodies for POLG and GAPDH. G: Quantification of Western blots in panel F, with protein levels expressed as a ratio to GAPDH and normalized to the mean of both control lines. Data are expressed as means ± SD. n = 3 independent differentiation experiments (B, C, E, and G). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. Scale bars = 25 μm (A and D).