Fig. 3.

Ames mutagenicity assay results for bacterial strains exposed to 1R6F cigarette smoke and regular- and menthol-flavored DT3.0a aerosol collected mass (ACM), total particulate matter (TPM), and gas-vapor phase (GVP) samples. Exposure to the (A–E) ACM/TPM and (F–J) GVP samples was performed using the pre-incubation method with metabolic activation. After 48 h of incubation, the numbers of revertants in (A and F) TA98, (B and G) TA100, (C and H) TA1537, (D and I) TA1535, and (E and J) TA102 were counted. Each result is the mean and standard error for triplicate independent tests. The concentrates where evidence of toxicity was observed as indicated with open marker is excluded in the assessment.