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. 2023 Feb 2;26(3):106123. doi: 10.1016/j.isci.2023.106123

Figure 2.

Figure 2

Distinct subgroups of 5-HT neurons mediate sexual receptivity

(A–D) Expression pattern of SPN (A) and the behavioral changes when the SPN were silenced with TNT (B-D). (A) Immunofluorescence of SPN (green) in a female SPNsplit-Gal4>UAS-mCD8::GFP. nc82 (magenta) serves as a counterstaining. (B and C) Quantification of the copulation rate (B) and latency to copulation (C) when SPNsplit-Gal4>UAS-TNT females were paired with wild-type males (n = 34-35). (D) The frequencies of pauses of the SPNsplit-Gal4>UAS-TNT females and control females toward the pursuing wild-type males (n = 30-31).

(E–H) Expression pattern of MB002B neurons (E) and the behavioral changes when the neurons were silenced with TNT (F-H). (E) Immunofluorescence of MB002B neurons (green) in a female of MB002B-Gal4>UAS-mCD8::GFP. nc82 (magenta) serves as a counterstaining. (F and G) Quantification of the copulation rate (F) and latency to copulation (G) when MB002B>UAS-TNT females were paired with wild-type males (n = 45–63). (H) Frequency of immobilization of the MB002B>UAS-TNT females and control females toward the pursuing wild-type males (n = 29–31).

(I–K) Quantification of courtship behaviors after SPN were activated by optogenetics for 80s.

(I and J) Quantification of the copulation rate (I) and latency to copulation (J) when SPNsplit-Gal4>UAS-PACα females were paired with wild-type males (n = 62–73).

(K) The pauses per minute of the SPNsplit-Gal4>UAS-PACα females toward the pursuing wild-type males (n = 62-73).

All genotypes and experimental conditions are indicated with the plots. In (I), for simplicity, only a portion of data points was visualized whereas the overall curve was derived from the entire dataset. In the box-and-whisker plot, the whiskers mark the minimum and maximum, the box includes the 25th to 75th percentiles, and the line within the box indicates the median of the dataset. The log-rank test was applied to (B), (F), and (I). Among the statistical comparisons between the experimental group and controls, only the ones with less significance are shown in (B) and (F). In (I), the statistical comparison was shown between the experimental group (with light) and the no-light control (with the same genotype). The Kruskal–Wallis test was performed for (C)-(D), (G)-(H), and (J)-(K). ∗p< 0.05; ∗∗p< 0.01; ∗∗∗p< 0.001; ∗∗∗∗p< 0.0001. Scale bar in (A) and (E): 100 μm.