REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-nc82 (mouse)	DSHB	CAT# nc82; RRID:AB_2314866
Rhodamine (TRITC) Conjugated Goat anti-Mouse IgG (H+L)	ZSGB-BIO	CAT# ZF 0313; RRID:AB_2571577
Rhodamine (TRITC) Conjugated Goat anti-Rabbit IgG (H+L)	ZSGB-BIO	CAT# ZF 0316; RRID:AB_2728778
HA-Tag (rabbit)	Cell Signaling	CAT# 3724; RRID:AB_1549585
Chemicals, peptides, and recombinant proteins
Serotonin hydrochloride	Alfa	CAT# B21263
Sex peptide	Sangon Biotech	MW: 4348.03 Lot No.: P22185-20210207
Adenosine 5′-triphosphate disodium salt hydrate	Sigma	CAT# A2383-5G
NaCl	Sinopharm Chemical Reagent Co.,Ltd	CAT# 10019308
KCl	XiLONG SCIENTIFIC	CAS# 7447-40-7
CaCl2	Sinopharm Chemical Reagent Co.,Ltd	CAT# 10005861
MgCl2.6H2O	Sinopharm Chemical Reagent Co.,Ltd	CAT# 10012892
NaHCO3	Sinopharm Chemical Reagent Co.,Ltd	CAT# 10018992
NaH2PO4.2H2O	XiLONG SCIENTIFIC	CAS# 13472-35-0
Trehalose	Sigma-Aldrich	CAT# T9531-10G
Sucrose	Sigma-Aldrich	CAT# V900116-500G
HEPES	Sigma-Aldrich	CAT# V900477-100G
Experimental models: Organisms/strains
Drosophila: Wildtype Canton S	Li Liu	RRID: DGRC_ 105666
Drosophila: Tph-Gal4	Yi Rao	Park et al., 200635
Drosophila: UAS-PACα	Benjamin Kottler and Martin Schwarzel	Bellmann et al., 201065
Drosophila: UAS-mCD8::GFP	Bloomington Drosophila Stock Center	RRID: BDSC_32185
Drosophila: Tub-Gal80ts	Yan Li and Aike Guo	McGuire et al., 200437
Drosophila: Crz-Gal4	Bloomington Drosophila Stock Center	RRID: BDSC_51976
Drosophila: UAS-Trh	Bloomington Drosophila Stock Center	RRID: BDSC_27638
Drosophila: UAS-Trh-RNAi	Julie H. Simpson	Albin et al., 201543
Drosophila: UAS-TNTE	Yan Li and Aike Guo	Sweeney et al., 199536
Drosophila: UAS-Kir2.1	Li Liu	Baines et al., 200145
Drosophila: 5-HT7Gal4	Yi Rao	Qian et al., 201757
Drosophila: 5-HT7-LexA	Yi Rao	Deng et al., 201966
Drosophila: 5-HT7-Gal4	Charles D Nichols	Becnel et al., 201129
Drosophila: UAS-Denmark	Yan Li and Aike Guo	RRID: BDSC_33061
Drosophila: trans-Tango	Bloomington Drosophila Stock Center	RRID: BDSC_77124
Drosophila: 20XUAS-IVS-GCaMP6m (attP40)	Bloomington Drosophila Stock Center	RRID: BDSC_42748
Drosophila: SPN split-Gal4	Thomas Preat	Scheunemann et al., 201844
Drosophila: MB002B	Bloomington Drosophila Stock Center	RRID: BDSC_68305
Drosophila: LexAop-GCaMP6f, UAS-P2X2	Fang Guo and Chang Liu	N/A
Software and algorithms
Prism9	GraphPad Software	URL: http://www.graphpad.com/;RRID:SCR_002798
MATLAB R2018a	MathWorks	URL: http://www.mathworks.com/products/matlab/; RRID:SCR_001622
Fiji	NIH	URL: https://fiji.sc/;RRID: SCR_002285
Adobe Illustrator	Adobe	URL: https://www.adobe.com/;RRID:SCR_010279
Deposited data
Raw and analyzed data	This paper; Mendeley Data	Mendeley Data: https://doi.org/10.17632/9y236v53m4.1