Figure 8.

In crystallo visualization of WT Pol η rU extension misincorporation with Mn²⁺.A–C, structures of WT Pol η during in crystallo catalysis after 10 mM Mn²⁺ soaking for 30s (B) and 180s (C). The 2F_o-F_c map for the primer terminus up conformation (blue) was contoured at 1.2 σ. The 2F_o-F_c map for the Me²⁺_A, Me²⁺_B, dGTP, and catalytic residues (blue) was contoured at 2.0 in A, 2.5 in B, and 2.0 σ in C. The F_o-F_c omit map for the down conformation of the primer terminus (green) was contoured at 3 σ in A–C. The F_o-F_c omit map for all the Mn²⁺_C, and newly formed bond (red) was contoured at 3.5 σ. D, timescale of reaction product of Pol η rU extension misincorporation in crystallo with Mn²⁺. Pol η, DNA polymerase η; rU, ribose uridine; dGTP, deoxyribose guanine triphosphate.