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. 2023 Jan 19;299(3):102923. doi: 10.1016/j.jbc.2023.102923

Table 2.

Summary of GSL-derived oligosaccharides of the neutral GSL fractions released by rEGCase II and identified by LC/ESI-MS2 in pooled tumor and normal pancreatic tissues of patients with pancreatic ductal adenocarcinoma

Trivial name
(Abbreviation)		 Oligosaccharide sequence Pooled tissues
 m/z
of
[M-H]- 		Retention time∗ [min]
T N T N
PX2 penta PX2-5 GalNAcβ3 Galβ4 GlcNAcβ3 Galβ4 Glc + - 909.3 24.5 --- --- ---
P1 penta P1-5 Galα4 Galβ4 GlcNAcβ3 Galβ4 Glc + - 868.3 26.9 --- --- ---
Neolactotetra nLc4 Galβ4 GlcNAcβ3 Galβ4 Glc + + 706.3 20.9 --- 20.7 ---
Neolactohexa nLc6 Galβ4 GlcNAcβ3 Galβ4 GlcNAcβ3 Galβ4 Glc + + 1071.4 27.0 27.6 26.9 27.6
H type 1 penta H5-1 Fucα2 Galβ3 GlcNAcβ3 Galβ4 Glc + + 852.3 19.4 20.5 19.3 20.4
H type 2 penta H5-2 Fucα2 Galβ4 GlcNAcβ3 Galβ4 Glc - + 852.3 --- --- 21.5 22.2
Lewisa penta Lea-5 Galβ3 (Fucα4) GlcNAcβ3 Galβ4 Glc + - 852.3 16.3 16.9 --- ---
Lewisx penta Lex-5 Galβ4 (Fucα3) GlcNAcβ3 Galβ4 Glc + + 852.3 16.2 --- 16.0 ---
Lewisb hexa Leb-6 Fucα2 Galβ3 (Fucα4) GlcNAcβ3 Galβ4 Glc + + 998.3 15.4 16.1 15.2 15.9
Lewisy hexa Ley-6 Fucα2 Galβ4 (Fucα3) GlcNAcβ3 Galβ4 Glc + + 998.3 17.9 --- 17.7 ---
Lewisx hepta Lex-7 Galβ4 (Fucα3) GlcNAcβ3 Galβ4 GlcNAcβ3 Galβ4 Glc + - 1217.4 22.5 23.0 --- ---
A type 2 hexa A6-2 GalNAcα3 (Fucα2) Galβ4 GlcNAcβ3 Galβ4 Glc - + 1055.3 --- --- 16.0 16.8
B type 2 hexa B6-2 Galα3 (Fucα2) Galβ4 GlcNAcβ3 Galβ4 Glc + + 1014.3 16.4 17.3 16.3 17.1
B type 2 hepta B7-2 Galα3 (Fucα2) Galβ4 (Fucα3) GlcNAcβ3 Galβ4 Glc + + 1160.3 14.1 --- 13.9 ---

A + sign stands for ′detected′, while a - sign stands for ′not detected′. T denotes tumor tissue and N denotes normal tissue.

∗ if two retention times are stated, it means that these oligosaccharides chains appeared as double peaks.