Table 4.
Carbohydrate-binding ligands used in chromatogram-binding assays
| Antibodies | Clone/ Designation |
Manufacturer/ Reference |
Dilution | Isotype | Binding specificity |
|---|---|---|---|---|---|
| Anti-blood group A | HE-193 | GeneTex/Abcam | 1:500 | IgM | GalNAcα3(Fucα2)Gal |
| Anti-Lewisa | 7LE | GeneTex/Abcam | 1:100 | IgG | Galβ3(Fucα4)GlcNAc |
| Anti-Lewisb | T218 | Santa Cruz Biotechnology | 1:200 | IgM | Fucα2Galβ3(Fucα4)GlcNAc |
| Anti-Neu5Ac-nLc4c | LM1:1a | (98) | 1:500 | IgM | Neu5Acα3Galβ4GlcNAc |
| Anti-Neu5Ac-Lc4c | TR4/SL-50 | (98) | 1:500 | IgM | Neu5Acα3Galβ3GlcNAc |
| Anti-Neu5Ac-Lea | 116-NS-19-9 | Signet | 1:50 | IgG1 | Neu5Acα3Galβ3(Fucα4)GlcNAc |
| Anti-Neu5Ac-Lex | KM93 | Merck | 1:50 | IgM | Neu5Acα3Galβ4(Fucα3)GlcNAc |
| P-fimbriated Escherichia coli | Strain 291-15 |
(74) | − | − | Galα4Gal |
| Erythrina crista-galli lectin | − | Vector Laboratories Inc. | 1:100 | − | Galβ4GlcNAc |
| Griffonia simplicifolia lectin IB4 | − | Advanced Targeting System | 1:200 | − | Galα |
c
This antibody was a kind gift of Dr. Maria Blomqvist, Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.