Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 1;20:54. doi: 10.1186/s12974-023-02744-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — Berberine increases hippocampal DG and CA1 subregional synaptic plasticity and adult neurogenesis induced by CORT. a Representation of the timing of experiments and illustration of the image of the target of PFC and hippocampus. b Hippocampal DG images show the decreasing signal intensity of PSD95 and SYN in the CORT group, and both BBR100 and BBR200 increased the PSD95 + and SYN + cell number (One-way ANOVA, F (3, 8) = 10.12, P = 0.0043; Dunnett’s multiple comparisons test, CORT vs. Control, P = 0.0021, CORT vs. BBR100, P = 0.0251, CORT vs. BBR200, P = 0.0081, n = 3 in each group). Scar bar, 100 µm. c Representation of the immunofluorescence labeling of hippocampal CA1, CORT significantly decreased the PSD95 + and SYN + cell number compared to BBR100 and BBR200 (One-way ANOVA, F (3, 8) = 84.63, P < 0.0001; Dunnett’s multiple comparisons test, all comparisons P < 0.0001, n = 3 in each group). Scar bar, 100 µm. d NeuN (red) and BrdU (green) in hippocampal DG images (One-way ANOVA, F (3, 8) = 155.0, P < 0.0001; Dunnett’s multiple comparisons test, all comparisons P < 0.0001, n = 3 in each group). Scar bar, 50 µm. e DAPI (blue) and DCX (red) in hippocampal DG images (One-way ANOVA, F (3, 8) = 220.0, P < 0.0001; Dunnett’s multiple comparisons test, all comparisons P < 0.0001, n = 3 in each group). Scar bar, 100 µm. f percentage of PSD95 + /SYN + area in hippocampal DG. g percentage of PSD95 + /SYN + area in hippocampal CA1. h BrdU + cell density in hippocampal DG. i DCX + cell density in hippocampal DG. j Western blotting assay for PSD95 and SYN, the CORT mice showed a significant difference in the expression of PSD95 and SYN (Two-way ANOVA, row factor F (1, 19) = 11.90, P = 0.0027, column factor F (3, 19) = 30.06, P < 0.0001; Dunnett’s multiple comparisons test, all comparisons P < 0.0001, n = 3 in each group). Bar graphs show the mean ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001. CORT corticosterone, BBR berberine, ANOVA analysis of variance, DG dentate gyrus, DCX Doublecortin, BrdU Bromodeoxyuridine, PSD95 postsynaptic density protein 95, SYN synapsin