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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Exp Cell Res. 2014 Dec 30;332(1):136–145. doi: 10.1016/j.yexcr.2014.12.009

Figure 1. Effect of PPase Inhibitors on MEK and Akt Phosphorylation and Expression.

Figure 1.

WEHI-231 cells were pre-treated with or without 1 or 10 nM Calyculin-A (Cal-A) or 100 nM Okadaic acid (OA) for 1h. The cells were then treated with or without 4 μM Manumycin-A (Man-A) for 120 min and pMEK/MEK, pAkt/Akt protein expression was determined by western blotting as described in the Materials and Methods. Treatment Lanes: 1, Untreated Control; 2, Man-A; 3, 1 nM Cal-A; 4, 1 nM Cal-A + Man-A; 5, 10 nM Cal-A; 6, 10 nM Cal-A + Man-A; 7, 100 nM OA; 8, 100 nM OA + Man-A. Arrows indicate cleaved MEK and Akt protein products. The results are representative of more than 4 separate, independent experiments.