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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Exp Cell Res. 2014 Dec 30;332(1):136–145. doi: 10.1016/j.yexcr.2014.12.009

Figure 5.

Figure 5.

Effect of Man on P-Akt, total Akt, P-PP1α/PP1α, and cleaved (activated) Caspase 3. Human prostate cancer (Du145 and LNCAP) and murine lymphoma (WEHI-231 and A20.2J) cell lines were maintained in exponential growth phase and were treated with or without 4 μM Manumycin-A for 0 (−) or 2h (+). Proteins were analyzed by western blot as described above. Note1: cells producing the data shown in rectangle with thick lines are more resistant to Man-A. Note2: Akt loss occurs only in WEHI-231. The data are representative of at least 3 separate, independent experiments.