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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Exp Cell Res. 2014 Dec 30;332(1):136–145. doi: 10.1016/j.yexcr.2014.12.009

Figure 6. Stable Transfection With Constitutively- Active PP1αT320A cDNA Sensitizes A20.2J To Man-A Mediated Apoptosis.

Figure 6.

A20.2J were transfected with either 5 μg pcDNA3 or 5 μg PP1αT320A/pcDNA3 then stably selected with G-418. Stably-selected cells were treated with or without 4 μM Man-A for 6h. Superoxide production and apoptosis were measured using DHE and propidium iodide fluorescent reporters as described above. The data are expressed as Experimental/Control. Apoptosis in the control, untreated cells was 4.0%.