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. 2023 Mar 1;3(3):347–360. doi: 10.1158/2767-9764.CRC-22-0365

FIGURE 1.

FIGURE 1

OAd-MSC TLR4−/− as a model of silent cells. A, Heatmap showing the in vitro inflammatory secretome at 24 hours after infection with OAd. Mock-infected MSCs were used as control. Light blue represents the lowest expression while dark blue represents the highest expression. B, Quantification by integrated density of proinflammatory cytokines differentially secreted by OAd-MSC WT and OAd-MSC TLR4−/−. C, Quantification of secreted CXCL10 by ELISA. Two-way ANOVA followed by Tukey multiple comparisons test. **, P < 0.01. D, Protein–protein interaction map of differentially expressed cytokines generated by STRING protein network generator. Thickness of the lines represents the strength of the association between proteins. Colors of the nodes indicate the processes in which the proteins are involved: inflammation (blue), cellular process (red), or cell migration (green). E, Protein expression of phospho-Jun (pJun), phospho-Akt (pAkt), and Actin analyzed by Western blot analysis at 24 hours. F, Graph represents the NFκB activation at 24 hours expressed by luminescence. Two-way ANOVA followed by Tukey multiple comparisons test. **, P < 0.01. For the whole figure, stripped bars correspond to mock-infected MSCs while solid bars correspond to OAd-MSC (WT cells in blue; TLR4−/− cells in red).