Skip to main content
. 2023 Mar 1;3(3):347–360. doi: 10.1158/2767-9764.CRC-22-0365

FIGURE 3.

FIGURE 3

OAd-MSC WT and OAd-MSC TLR4−/− equally migrates to tumor cells in vitro. A, Transwell migration assay of MSCs and OAd-MSC toward CMT64 tumor cells at 24 hours (n = 30). DMEM alone as stimuli was used as negative control (n = 2). Migrated cells were quantified in 10X HPF. Two-way followed by Tukey multiple comparisons test. ns, not significant; ***, P < 0.001. B, Representative images of migrated cells. C, Representative images of migrating OAd-MSC (right) seeded in 2-well inserts in the presence of CMT64 tumor cells (left). Color lines represent in vivo tracking of individual OAd-MSC for 24 hours. D, Trajectory plot of tracked OAd-MSC (n = 38 in OAd-MSC WT, n = 48 in OAd-MSC TLR4−/−). E, Parameters measured from tracked cell trajectories of migrating OAd-MSC toward tumor cells.