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. 2021 Nov 26;14(1):e14485. doi: 10.15252/emmm.202114485

Figure 2. SPINK6 inhibits HAT and KLK5, and HAT‐ and KLK5‐mediated HA cleavage.

Figure 2

  1. At 48 h post‐transfection of the indicated protease plasmids in triplicate, BHK21 cells were incubated with a fluorogenic substrate and wtSPINK6 or loss‐of‐function mutSPINK6 or PBS for 2 h and then applied to fluorescence assay. Data represent mean and SD of triplicated wells in a representative experiment performed three times. **P < 0.01. Student’s t‐test.
  2. At 48 h after co‐transfection of the indicated protease plasmids and H7 or H1 plasmid, BHK21 cells were lysed to examine HA cleavage with WB.
  3. At 48 h after triple transfection of H7, the indicated proteases, and wtSPINK6 or vector, BHK21 cells were lysed for the detection of HA cleavage.
  4. At 48 h after triple transfection of H7, HAT or TMPRSS2, and wtSPINK6 or mutSPINK6, BHK21 cells were lysed for the detection of HA cleavage.
  5. At 48 h after triple transfection of H1, the indicated proteases, and wtSPINK6 or mutSPINK6, BHK21 cells were lysed for the detection of HA cleavage.

Source data are available online for this figure.