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. 2021 Nov 26;14(1):e14485. doi: 10.15252/emmm.202114485

Figure 3. SPINK6 inhibits virus growth and maturation‐mediated HAT and KLK5.

Figure 3

  1. At 36 h after transfection of the indicted protease and wtSPINK6 plasmids or blank vector in triplicate, BHK21 cells were inoculated with H7N9/ah at a MOI of 0.5. Cell‐free culture media were harvested at 24 hpi for viral titration. Data represent the mean and SD of the triplicated wells in a representative experiment performed three times. **P < 0.01. Student’s t‐test.
  2. At 24 h after transfection of HAT or KLK5 plasmid in sextuplicate, A549 cells were inoculated with H1N1/pdm at MOI of 0.25. The infected cells were incubated with recombinant wtSPINK6 or mutSPINK6 protein in triplicate for 24 h. Culture media in each well were stored in two aliquots, one was applied to viral titration with conventional plaque assay (black bars) and the other was pre‐treated with TPCK trypsin for 1 h prior to plaque assay (grey bars). Data represent the mean and SD of the triplicated wells in a representative experiment performed three times. *P < 0.05; **P < 0.01; ***P < 0.001. Student’s t‐test.