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Small mycelia cultures are ideal for nucleic acid extractions. (A) Inoculate 2× GYE in a vented 50-ml conical tube. (B) Incubate at 30°C for 5 to 7 days. (C) Healthy cultures are fluffy, rather than stringy. (D) Pour mycelia through a nested filter. (E) Rinse fungal material with PBS until white.
