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Spherule growth. (A) Culture arthroconidia at ~1 × 106 cells/ml in RPMI-sph medium at 39°C, 10% CO2, for ≥3 days. (B) Pour cultures into a 50-ml conical tube and pellet cells by centrifugation. (C) After resuspension of the pellet, confirm the presence of spherules. Shown is C. posadasii Silveira on day 4, after growth in RPMI-sph medium. (D) Packets of endospores are present after medium change. Shown is C. posadasii Silveira on day 7 after changing the RPMI-sph medium on day 4.
