Table 1.
Stock Solutions for 1 L Modified Converse Medium
| Reagenta | MW | g/L | Molarity or concentration |
Volume/1 Lb |
|---|---|---|---|---|
| Ammonium acetate | 77.08 | 123.0 | 1.596 M | 10 ml |
| KH2PO4 anhydrousc | 136.09 | 51.0 | 0.37 M | 10 ml |
| K2HPO4 anhydrousc | 174.18 | 52.3 | 0.3 M | 10 ml |
| MgSO47H2Oc | 246.5 | 39.5 | 0.16 M | 10 ml |
| ZnSO47H2Oc | 287.56 | 0.72 | 2.5 mM | 5 ml |
| NaCl | 58.44 | 1.4 | 24 mM | 10 ml |
| CaCl22H2Oc | 147.02 | 0.6 | 4.08 mM | 5 ml |
| NaHCO3 | 84.01 | 1.2 | 14.3 mM | 10 ml |
| Tamold | NA | 50.0 | 50 g/L | 10 ml |
| Glucosee | 180.86 | 40.0 | 40 g/L | 100 ml |
| N-Z aminee | NA | 5.0 | 5 g/L | 10 ml |
Prepare each stock solution in 1 L bottled water (distilled deionized water in clean glassware) and number as shown above. The stocks may be stored ≤1 year at 4°C.
Allocate 820 ml distilled deionized water into a container for solid medium and 810 ml for liquid medium. Pipet individual reagents into the flask, swirling to mix after each addition. pH to 6.5. Autoclave for 30 min. For solid medium, add 18 g/L purified agar. When cool, add sterile-filtered glucose and N-Z amine if making liquid medium and only sterile-filtered glucose if making solid Converse plates.
The hydrated form of these particular reagents can be substituted, but the weight will need to be adjusted to obtain the correct molarity.
Omit from the medium for agar plates.
Filter-sterilize and add after autoclaving.