Table 1. Theoretical Number Average Molecular Weight (M_n), Number Average Hydrodynamic Diameter (D_h), Electrophoretic Mobility, and Apparent Zeta Potential (ζ) of Bottlebrush Polymers.

Sample	Mna (g mol–1)	Dh (nm)b	Electrophoresis mobility (μm cm V–1 s–1)c	ζ (mV)d
P[BiBEM-g-(PAA50-b-PNIPAm50)]320 (SBB50)	2.96 × 106	39.5 ± 5.4	–1.29 ± 0.04	–16.5 ± 0.6
P[BiBEM-g-(PAA50-b-PNIPAm150)]320 (SBB150)	6.58 × 106	41.8 ± 1.3	–0.82 ± 0.06	–10.5 ± 0.7
P[BiBEM-g-(PAA50-b-PNIPAm50)]1600 (LBB50)	1.53 × 107	105.5 ± 5.3	–1.26 ± 0.05	–15.4 ± 0.6
P[BiBEM-g-(PAA50-b-PNIPAm150)]1600 (LBB150)	3.34 × 107	91.5 ± 4.9	–0.71 ± 0.09	–9.1 ± 1.2

^aTheoretical number average molecular weight calculated according to the chemical composition.

^bHydrodynamic diameters were determined in water at 100 mg L^–1 of polymer concentration at pH 6.5 (10 mM NaCl) by dynamic light scattering (Malvern Zetasizer Nano ZS). Bottlebrush size distributions are shown in Figure S3.

^cElectrophoretic mobility measured with 100 mg L^–1 of polymer solution at pH 6.5 with 10 mM NaCl (Malvern Zetasizer Nano ZS).

^dApparent zeta potentials were calculated from the mobility using the Smoluchowski model.