. Author manuscript; available in PMC: 2023 Nov 1.

Published in final edited form as: Neurobiol Dis. 2022 Sep 29;174:105878. doi: 10.1016/j.nbd.2022.105878

Table 1:

Use of retrograde viral vectors to map connectivity to the striatum

Study and species	Viral vector^*	Animals	Injection site	Type of neuron targeted	Main sources of cortical inputs identified	Thalamic afferents identified
Studies using rabies virus to achieve trans-synaptic followed by retrograde tracing
(Wall et al. 2013)	AAV-EF1a-FLEX-GTB or AAV-FLEX-hGTB; (EnvA)-deltaG- Rabies	D1R-Cre mice	Dorsal striatum (tendency to lateral half)	dSPNs	S1, S2, M1, M2, insular, orbitofrontal; cingulate	PF main source, non-PF contribute
(Wall et al. 2013)	AAV-EF1a-FLEX-GTB or AAV-FLEX-hGTB; (EnvA)-deltaG- Rabies	D2R-Cre mice	Dorsal striatum (tendency to lateral half)	iSPNs	S1, S2, M1, M2, insular, orbitofrontal	PF main source, non-PF
(Guo et al. 2015)	AAV-DIO -TVA and AAV-DIO-RG; (EnvA)-deltaG-Rabies	D1R-Cre mice	Dorsomedial striatum	dSPNs	Cingulate, M1, M2, S1	PF, non-PF
		D2R-Cre mice		iSPNs	Cingulate, M1, M2, S1	PF, non-PF
		Chat-Cre mice		ChINs	Cingulate, M2	PF, non-PF
(Smith et al. 2016)	AAV5-EF1a-Flex-TVA and AAV8-CA-Flex-RG; (EnvA)-deltaG-Rabies	Plxnd1-OG1 (matrix-Cre) mice	Dorsal striatum (no distinction medial vs lateral)	Matrix (preferentially SPNs)	More limbic and sensory cortical inputs to matrix than patch, but both compartments receive inputs from all areas	PF, non-PF
(Smith et al. 2016)		Sepw1-NP67 (patch-Cre) mice	Dorsal striatum (no distinction medial vs lateral)	Patch (preferentially dSPNs)		PF, non-PF
(Klug et al. 2018)	AAV5-TVA: AAV8-CA-RG; (EnvA)-deltaG-Rabies	ChAT-IRES-Cre mice	Dorsal striatum (no distinction medial vs lateral)	ChINs	Cingulate, M2, S1	PF
(Klug et al. 2018)	AAV5-TVA: AAV8-CA-RG; (EnvA)-deltaG-Rabies	PV-Cre mice	Dorsal striatum (no distinction medial vs lateral)	PV	Cingulate, M1, M2, S1	Non-PF
(Choi et al. 2019)	AAV5-CAG-DIO-TVA; AAV8-CAG-DIO-G; (EnvA)-deltaG-rabies	Drd1a-Cre mice	Dorsomedial striatum	dSPNs	Cingulate, Orbitofrontal, M2, M1, S1	PF main source, non-PF contribute
		PV-2a-Cre mice		PV	Cingulate, Orbitofrontal, M2, M1, S1, retrosplenial	PF main source, non-PF contribute
		SST-IRES-Cre mice		SST+ LTSIs.	Cingulate, Orbitofrontal, M2, M1, S1	PF main source, non-PF contribute
(Melendez-Zaidi et al. 2019)	AAV9-EF1a-DIO-HTB; (EnvA)-deltaG-rabies	SST-IRES-Cre	Dorsal striatum	LTSIs	Cingulate, M2	PF
Studies using AAVretro (AAVrg) to achieve retrograde tracing
(Tervo et al. 2016)	AAVrg-hSyn-Cre	Rosa26-LSL-H2B-EGFP mice	Dorsomedial striatum	Unspecific	Perirhinal, ectorhinal temporal, auditory	PF, non-PF
(Weiss et al. 2020)	AAVrg-CMV	Rhesus macaques	Caudate nucleus (head) and putamen	Unspecific	Prefrontal, orbitofrontal, premotor, cingulate, supplemental motor, M1, insular	Non-PF^**
(Albaugh et al. 2020)	AAVrg-hSyn	Rats	Dorsal striatum	Unspecific	Mulitple cortical regions	PF, non-PF
(Albaugh et al. 2020)	AAVrg-hSyn	Rhesus macaques	Putamen	Unspecific	Multiple cortical regions, most prominent in frontal regions.	Negligible^**

For simplicity the reporter proteins used are not mentioned in this table

^**

Note that the lack of thalamostriatal inputs identified with AAVrg in rhesus macaques contrasts with previous literature findings, and likely indicates a pathway selectivity of the vector. See main text for further details.