Fig. 15.

CLR01 and CLR05 disrupt S. aureus biofilm in distinct manners. (A) 50 µ M CLR01, CLR03, or CLR05 were added to S. aureus cultures at t = 0. Biofilm images show viable cells stained in green using the LIVE/DEAD BacLight Bacterial Viability Kit. The images were recorded after 24 h of incubation using confocal fluorescence microscopy (excitation/emission: 485/498 nm). Scale bars, 50 mm. (B) TEM micrographs of PSMα1 aggregated in the absence or presence of equimolar MT concentrations results in short scarce fibrils in the presence of CLR01 and quasi-oval multiple fibril bundles in the case of CLR05. (C) TEM micrographs testing potential disaggregation of preformed PSMα1 fibrils by CLR01 or CLR05. The MTs were added at a 1:1 concentration ratio and incubated for 24 hours at 37°C. Scale bars denote 300 nm. Reprinted by permission from Elsevier License: Elsevier, Cell Chemical Biology, Inhibition of Staphylococcus aureus biofilm-forming functional amyloid by molecular tweezers, Malishev R, Salinas N, Gibson J, Eden A, Mieres-Perez J, Ruiz-Blanco Y, Malka O, Kolusheva S, Klärner F, Schrader T, Sanchez-Garcia E, Wang C, Landau M, Bitan G, Jelinek R (2021).