Fig. 18.

MTs are internalized in SH-SY5Y cells and concentrate in puncta. SH-SY5Y cells were incubated with fluorescent MTs for 24 hours and visualized by fluorescence microscopy. (A) Cells were transiently transfected with GFP-actin (green), incubated with 5 µM CLR16 (red), and nuclei were stained with Hoechst (blue). (B) Cells were incubated with 10 µM CLR18 (green) and nuclei were stained with Hoechst (blue). This figure was published originally in Communications Biology, Li, Z., Siddique, I., Hadrovic, I., Kirupakaran, A., Li, J., Zhang, Y., Klärner, F-G., Schrader, T., Bitan, G. Lysine-selective molecular tweezers are cell penetrant and concentrate in lysosomes, 165513, Copyright Nature Portfolio (2021).