Fig. 6.

CLR01 binding induces a massive upfield shift of Lys sidechain protons inside its cavity. ¹H-NMR spectra were recorded for CLR01, IAPP(1 − 7), and their 1:1 or 1:3 complexes in 10 mM phosphate-buffered D₂O, pH 7.2. The Lys1 side-chain methylene signals are assigned as α, β, γ, δ, and ε. All the methylene resonances, most notably the δ- and ε-protons, are shifted upfield by 4.4 and 4.5 ppm, respectively, indicating strong binding of CLR01 to Lys1.