Fig. 9.

CLR01 inhibits α-syn assembly and disaggregates α-syn in vitro. (A) β-Sheet formation in α-syn in the absence or presence of different concentrations of CLR01 or CLR03 was followed by measuring ThT fluorescence. (B) Electron micrographs of α-syn in the absence or presence of 10-fold molar excess of CLR01 or CLR03 at the end of the reactions shown in panel A. Scale bars denote 100 nm. (C) Disaggregation of α-syn fibrils by CLR01 was initiated at two time points: On day 8 (dissociation reaction D1) or on day 24 (D2), and the reactions were monitored using ThT fluorescence. Electron micrographs were obtained periodically and show the morphology of α-syn at the indicated time points. Scale bars denote 100 nm. The data are an average of three independent experiments. Reprinted by permission from Springer Nature Customer Service Centre GmbH: Springer Nature, Neurotherapeutics, A novel “molecular tweezer” inhibitor of α-synuclein neurotoxicity in vitro and in vivo, Prabhudesai, S., Sinha, S., Attar, A., Kotagiri, A., Fitzmaurice, A. G., Lakshmanan, R. Ivanova, M. I., Loo, J. A., Klärner, F. G., Schrader, T., Stahl, M., Bitan, G., and Bronstein, J. M. (2012).