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. 2023 Jan 30;51(4):1571–1582. doi: 10.1093/nar/gkad004

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Analysis of single-molecule FRET time-traces. Results of HMM fitting are shown by red lines. (A, B) Representative FRET time-traces from the two main group classifications, respectively. Dashed line marks E= 0.6. Specific FRET states corresponding to mostly unfolded (1) as well as partially and fully folded DNA conformations (A, B and C) are marked by arrows. (C) Representative FRET time-trace of the small population of the high FRET group that features transitions from the lowest to the highest FRET state (event marked by a black arrow). (D) FRET histogram showing single-molecule data from the low (gray with no edge) and high (gray with black edge) FRET groups.