Table 1.
Biophysical parameters derived from SAXS and MALS data
| Instrument = ALS beamline 12.3.1; q range = 0.01–0.35 (Å−1); exposure time = 3 s; temperature = 20°C | ||||||||
|---|---|---|---|---|---|---|---|---|
| Parameters | Full length (FL) | NT + ZF | ZF + CT | DNA | ||||
| No DNA | + DNA | No DNA | + DNA | No DNA | + DNA | |||
| Concentration | (mg/ml) | 4 | 4 | 5 | 5 | 13 | 13 | 5 |
| (μM) | ∼77 | ∼124 | ∼448 | ∼454 | ||||
| MW (kDa) | Calculated (monomer) | 52 | 63 | 40 | 51 | 29 | 40 | 11.1 |
| SEC-MALS | 56 | 63 | 46 | 52 | 33 | 40 | 11.2 | |
| SAXS MoW | 55.9 | 64 | 43.7 | 53.8 | 31.7 | 38.1 | 10.8 | |
| Porod volume (Å3) | 105 839 | 117 525 | 78 713 | 94 146 | 55 180 | 70 985 | 15 604 | |
| D max (Å) | 122 | 142 | 107 | 141 | 95 | 119 | 58 | |
| Low-q range (Å−1) used for Guinier analysis | 0.02–0.04 | 0.02–0.03 | 0.01–0.05 | 0.01–0.03 | 0.02–0.05 | 0.01–0.04 | 0.02–0.07 | |
| I(0) (Å) | from Guinier | 8.41 | 21.81 | 4.72 | 30.28 | 9.79 | 130.50 | 25.90 |
| from P(r) | 8.41 | 21.81 | 4.72 | 30.28 | 9.78 | 130.50 | 25.91 | |
| R g (Å) | from Guinier | 38.54 | 43.80 | 31.95 | 40.60 | 29.71 | 30.72 | 18.33 |
| from P(r) | 38.69 | 43.85 | 31.11 | 40.62 | 29.71 | 30.89 | 18.38 | |
Values of Rg and I(0) parameters were estimated from Guinier plot and P(r) plot analyses.