Fig 2. Reporter transgene test of 18 predicted CREs from the first dorsal pupal abdomen CRE training set.
(A and C-N) EGFP reporter transgene expressions in the dorsal abdomens of male D. melanogaster pupa at ~80–88 hours after puparium formation (hAPF) or the P13-P14(i) developmental stage. Expressions in the female abdomens were similar and therefore not reported here. (A) In the absence of a CRE, EGFP expression was seen in some abdominal muscles, but little to no expression was seen in the abdominal epidermis. (B) Of the 18 pCREs tested by reporter transgene assays, twelve showed reproducible dorsal abdominal epidermis expression that exceeds what is observed in the (A) no CRE control. (C-N) Among the pCREs with abdominal expression regulatory activity, EGFP expression was generally seen throughout abdominal segments A2-A6. All reporter transgenes were inserted into the 51D attP site on the 2nd chromosome, and therefore experience the same genomic and chromatin environments. (J) Brightness was increased in Adobe Photoshop by 25% compared to the other figure panels with EGFP expressions.