Fig 3. Non-coding sequences selected solely for the possession of similar nucleotide compositions to a specific training set CRE failed to drive noteworthy pupal abdominal epidermis expression.
(A-E) EGFP reporter transgene expressions in the dorsal abdomens of male D. melanogaster pupa at ~80–88 hours after puparium formation (hAPF) or the P13-P14(i) developmental stage. Expressions in the female abdomens were similar and therefore not reported here. (A) EGFP expression pattern when the reporter transgene does not possess a CRE. (B) Expression driven by an X-chromosome non-coding sequence (X:7749947–7751734) with nucleotide composition equivalent to the Dopa decarboxylase Male Epidermis Element 1 or “Ddc_MEE1” CRE. (C) Expression driven by a 2nd chromosome non-coding sequence (2L:11922488–11924519) with nucleotide composition equivalent to the bric à brac (bab) “anterior element” (ACEsub3 region) CRE. (D) Expression driven by a 3rd chromosome non-coding sequence (3L:27532906–27534457) with nucleotide composition equivalent to the ebony activation element CRE. (E) EGFP expression driven by the S1.1 CRE is shown to illustrate the activity of a sequence with the genuine pupal abdomen regulatory activity. 3A and 3E are redundant images to those used in Fig 2A and 2C respectively.