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. 2023 Jan;33(1):1–17. doi: 10.1101/gr.276643.122

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© 2023 Islam et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Transcriptional activity of promoters does not regulate nongenic transcription at boundaries. (A) Browser shots displaying the promoters targeted by CRISPRi (black arrows); the track is overlaid with CTCF, H3K4me3, H3K27ac, and mNET-seq tracks. (B) qRT-PCR assessing the changes in INK4a/ARF mRNA, sense, and antisense boundary RNA arising from B1 and B2 CTCF sites upon CRISPRi on promoters. (C) CTCF enrichment on boundaries of INK4a/ARF and its flanking TADs upon CRISPRi on INK4a/ARF promoters. Error bars denote SEM from three biological replicates. P-values were calculated by the Student's two-tailed unpaired t-test in B and C. (**) P < 0.01, (*) P < 0.05, (ns) P > 0.05.