Figure 1.

A study of gonadal aging in the naturally short-lived African turquoise killifish. (A) Experimental scheme. Killifish gonads (N = 5 per group) were harvested, and only high-quality RNA samples were further processed (see Supplemental Fig. S1A). (B) Lifespan curve for female and male GRZ strain killifish at our facility. GRZ females lived significantly longer than males (P = 0.002; log-rank test). Median lifespan: female, 26.6 wk; male, 19.4 wk. Vertical dotted lines indicate ages in this study (5, 10, 15 wk). (C–E) Principal component analyses (PCAs) for mRNA gene expression (C), TE expression (D), and TE-mapping piRNA abundance (E; see Methods).