Skip to main content
. 2023 Mar 1;9(9):eade3760. doi: 10.1126/sciadv.ade3760

Fig. 3. ATM-SPARK achieves spatial resolution and colocalizes with DNA damage markers and telomere DNA upon radiomimetic drug-induced DNA damage.

Fig. 3.

(A) Fluorescence images of cells upon laser irradiation on two spatially localized areas (circled by the white dashed lines). Cells were transfected with ATM-SPARK and mCherry-POLQ. (B) ATM-SPARK droplets were colocalized with mApple-53BP1 upon etoposide-induced DNA damage. a.u., arbitrary units. (C) ATM-SPARK droplets were colocalized with BRCA1-mCherry upon addition of etoposide. (D) ATM-SPARK droplets were associated with telomere DNA upon NCS-induced DNA damage. Scale bars, 5 μm (A and D) and 10 μm (B and C).