Table 2. Susceptibility results of various β-lactams against L1 variants expressed in the pBC SK (+) vector in E. coli DH10B cells.

The reported values are the mode of at least three biological replicates.

Strain	MIC value (mg/l)*
	CAZ	FEP	IMI	MER	TEB	TEB + CS319†
DH10B pBC SK blaL1a	256	2	16	16	32	2
DH10B pBC SK blaL1a_H151V	2	0.03	0.125	0.03	≤0.03	≤0.03
DH10B pBC SK blaL1a_D150cV	256	1	8	8	4	0.25
DH10B pBC SK blaL1a_P225A	64	0.125	0.25	2	0.125	≤0.03
DH10B pBC SK blaL1a_R236A	256	2	8	8	4	0.25
DH10B pBC SK blaL1a_Y227A	64	0.25	0.25	2	0.5	≤0.03

^*CAZ, ceftazidime; FEP, cefepime; IMI, imipenem; MER, meropenem; TEB, tebipenem.

^†CS319 was added to a final concentration of 100 mg/l.