Table 2. Comparison of ChrMP459 and HO-2 null effects on preBötC and XIIn transmission properties.
Properties of pharmacological effects of HO-2 dysregulation on preBötC and XIIn activity via ChrMP459 application were compared to genetic dysregulation in HO-2 null mouse experiments. Instantaneous frequency (finst), integrated burst amplitude, irregularity of integrated burst amplitude, and frequency of subnetwork bursting (% of total bursts) were analyzed for preBötC activity. I/O ratio of integrated preBötC area input to XIIn output and percent transmission were compared between the two regions. XIIn disinhibited neurons synaptic drive potentials were also assessed. Statistical analysis for all comparisons via unpaired t-test; values presented as mean ± SEM; significance level P<0.05.
| Metric | ChrMP459 (n) | HO-2 null (n) | p-value |
|---|---|---|---|
| finst* (Hz) | 0.24±0.019 (34) | 0.42±0.049 (11) | <0.0001 |
| Burst Amplitude* (mV) | 0.08±0.012 (34) | 0.05±0.010 (11) | 0.189 |
| IrS AMP† (A.U.) | 0.41±0.050 (34) | 0.39±0.070 (11) | 0.127 |
| Subnetwork‡ (%) | 19.43±3.373 (34) | 16.70±4.898 (11) | 0.670 |
| I/O Ratio§ (A.U.) | 0.59±0.064 (34) | 0.79±0.074 (11) | 0.096 |
| Transmission¶ (%) | 75.10±3.425 (34) | 64.11±6.002 (11) | 0.119 |
| Synaptic Drive Current** (pA) | –95.31±21.790 (19) | –36.71±2.141 (8) | 0.097 |
Data originally reported in Table 1.
data from Figure 5B and Figure 8—figure supplement 1.
data from Figure 1D and Figure 9—figure supplement 1.
data from Figure 1E and Figure 9—figure supplement 1.