. 2023 Feb 28;12:e81302. doi: 10.7554/eLife.81302

Appendix 1—key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Gene (Homo sapiens)	eIF4A1	NCBI	GenBank:CCDS11113.1
Gene (Ophiocordyceps sinensis)	eIF4A	EnsemblFungi (https://fungi.ensembl.org/index.html)	EnsemblFungi:OCS_04979
Gene (Ophiocordyceps sp. BRM1)	eIF4A iso4	This study		Iwasaki lab
Gene (Colletotrichum orbiculare)	eIF4A	GenBank	GenBank:Cob_v000942
Gene (C. orbiculare)	60S ribosomal protein L5	GenBank	GenBank:Cob_v012718
Gene (Cucumis sativus)	Cyclophilin	GenBank	GenBank:AY942800.1
Strain, strain background (Aglaia odorata)	Aglaia odorata	This paper		Grown in Berkeley, CA; Ingolia lab
Strain, strain background (Ophiocordyceps sp. BRM1)	Ophiocordyceps sp. BRM1	This paper		Grown in Berkeley, CA; Ingolia lab
Strain, strain background (Escherichia coli)	BL21 Star (DE3)	Thermo Fisher Scientific	Cat. #:C601003
Strain, strain background (C. orbiculare)	104-T	NARO GenBank	MAFF 240422
Strain, strain background (C. orbiculare)	eIF4A^WT#1	This paper	CoNK1171	Supplementary file 4; Shirasu lab
Strain, strain background (C. orbiculare)	eIF4A^WT#2	This paper	CoNK1172	Supplementary file 4; Shirasu lab
Strain, strain background (C. orbiculare)	eIF4A^H153G#1	This paper	CoNK1181	Supplementary file 4; Shirasu lab
Strain, strain background (C. orbiculare)	eIF4A^H153G#2	This paper	CoNK1182	Supplementary file 4; Shirasu lab
Strain, strain background (C. sativus)	Suyo strain	Sakata Seed Corp.
Recombinant DNA reagent	pColdI (plasmid)	TaKaRa	Cat. #:3361
Recombinant DNA reagent	pColdI-H. sapiens eIF4A1 WT (plasmid)	RIEN BRC	RDB17299	Iwasaki et al., 2019
Recombinant DNA reagent	pColdI-H. sapiens eIF4A1 Phe163Gly (plasmid)	This paper		Iwasaki lab
Recombinant DNA reagent	pColdI-H. sapiens eIF4A1 Phe163His (plasmid)	This paper		Iwasaki lab
Recombinant DNA reagent	pColdI-O. sinensis eIF4A WT (plasmid)	This paper		Iwasaki lab
Recombinant DNA reagent	pColdI-O. sinensis eIF4A His154Gly (plasmid)	This paper		Iwasaki lab
Recombinant DNA reagent	pColdI-Ophiocordyceps sp. BRM1 eIF4A iso4 WT (plasmid)	This paper		Iwasaki lab
Recombinant DNA reagent	pColdI-Ophiocordyceps sp. BRM1 eIF4A iso4 Gly172His (plasmid)	This paper		Iwasaki lab
Recombinant DNA reagent	pColdI-Ophiocordyceps sp. BRM1 eIF4A iso4 Gly172Phe (plasmid)	This paper		Iwasaki lab
Recombinant DNA reagent	pENTR4 (plasmid)	Thermo Fisher Scientific	Cat. #:A10465
Recombinant DNA reagent	pENTR4-C. orbiculare eIF4A WT (plasmid)	This paper		Shirasu lab
Recombinant DNA reagent	pENTR4-C. orbiculare eIF4A His153Gly (plasmid)	This paper		Shirasu lab
Recombinant DNA reagent	pII99 (plasmid)	Namiki et al., 2001
Recombinant DNA reagent	psiCHECK2−7×AGAGAG motifs	Iwasaki et al., 2016
Recombinant DNA reagent	psiCHECK2-CAA repeats	Iwasaki et al., 2016
Sequence-based reagent	Random Primer (nonadeoxyribonucleotide mix: pd(N)₉)	TaKaRa	Cat. #:3802
Sequence-based reagent	FAM-labeled [AG]₁₀ RNA	Iwasaki et al., 2019
Sequence-based reagent	Primers	This paper		Supplementary file 5; Shirasu lab
Peptide, recombinant protein	H. sapiens eIF4A1 WT	This paper		Iwasaki lab
Peptide, recombinant protein	H. sapiens eIF4A1 Phe163Gly	This paper		Iwasaki lab
Peptide, recombinant protein	H. sapiens eIF4A1 Phe163His	This paper		Iwasaki lab
Peptide, recombinant protein	O. sinensis eIF4A WT	This paper		Iwasaki lab
Peptide, recombinant protein	O. sinensis eIF4A His154Gly	This paper		Iwasaki lab
Peptide, recombinant protein	Ophiocordyceps sp. BRM1 eIF4A iso4 WT	This paper		Iwasaki lab
Peptide, recombinant protein	Ophiocordyceps sp. BRM1 eIF4A iso4 Gly172His	This paper		Iwasaki lab
Peptide, recombinant protein	Ophiocordyceps sp. BRM1 eIF4A iso4 Gly172Phe	This paper		Iwasaki lab
Peptide, recombinant protein	Driselase from Basidiomycetes sp.	Sigma-Aldrich	Cat. #:D9515
Peptide, recombinant protein	Lysing enzyme from Trichoderma harzianum	Sigma-Aldrich	Cat. #:L1412
Peptide, recombinant protein	Cas9 nuclease protein NLS	Nippon Gene	Cat. #:316-08651
Peptide, recombinant protein	Turbo DNase	Thermo Fisher Scientific	Cat. #:AM2238
Peptide, recombinant protein	RNase I	Lucigen	Cat. #:N6901K
Commercial assay or kit	rRNA depletion by a Ribo-Zero Gold rRNA Removal Kit (Yeast)	Illumina	Cat. #:RZY1324
Commercial assay or kit	TruSeq Stranded mRNA Kit	Illumina	Cat. #:15027078
Commercial assay or kit	In-Fusion HD	TaKaRa	Cat. #:639650
Commercial assay or kit	ProtoScript II Reverse Transcriptase	New England Biolabs	Cat. #:M0368L
Commercial assay or kit	HiFi DNA assembly	New England Biolabs	Cat. #:E2621
Commercial assay or kit	Ni-NTA agarose	QIAGEN	Cat. #:30230
Commercial assay or kit	HiTrap Heparin HP column, 1 ml	GE Healthcare	Cat. #:17040601
Commercial assay or kit	NAP-5	GE Healthcare	Cat. #:17085302
Commercial assay or kit	PD-10	GE Healthcare	Cat. #:17085101
Commercial assay or kit	Vivaspin 6 (10 kDa MWCO)	Sartorius	Cat. #:VS0601
Commercial assay or kit	EzStainAQua	ATTO	Cat. #:2332370
Commercial assay or kit	Black 384-well microplate	Corning	Cat. #:3820
Commercial assay or kit	T7-Scribe Standard RNA IVT Kit	CELLSCRIPT	Cat. #:C-AS3107
Commercial assay or kit	ScriptCap m⁷G Capping System	CELLSCRIPT	Cat. #:C-SCCE0625
Commercial assay or kit	ScriptCap 2′-O-Methyltransferase Kit	CELLSCRIPT	Cat. #:C-SCMT0625
Commercial assay or kit	A-Plus Poly(A) Polymerase Tailing Kit	CELLSCRIPT	Cat. #:C-PAP5104H
Commercial assay or kit	Renilla-Glo Luciferase Assay System	Promega	Cat. #:E2720
Commercial assay or kit	MicroSpin G-25 column	Cytiva	Cat. #:27532501
Commercial assay or kit	Rabbit Reticulocyte Lysate, Nuclease-Treated	Promega	Cat. #:L4960
Commercial assay or kit	Potato dextrose agar (PDA) medium	Nissui	Cat. #:05709
Commercial assay or kit	Potato dextrose broth	BD Biosciences	Cat. #:254920
Commercial assay or kit	70 µm cell strainer	Corning	Cat. #:352350
Commercial assay or kit	Yeast extract	BD Biosciences	Cat. #:212750
Commercial assay or kit	Filter (0.2 µm pore size)	GE Healthcare	Cat. #:6900-2502
Commercial assay or kit	50 ml tube	Falcon, Corning	Cat. #:352070
Commercial assay or kit	CUGA7 gRNA Synthesis Kit	Nippon Gene	Cat. #:314-08691
Commercial assay or kit	50 ml ProteoSave SS tube	Sumitomo Bakelite	Cat. #:631-28111
Commercial assay or kit	MF membrane (0.45 µm pore size)	Millipore	Cat. #:HAWP04700
Commercial assay or kit	TRIzol reagent	Thermo Fisher Scientific	Cat. #:15596018
Commercial assay or kit	Ribo-Minus Eukaryotes Kit for RNA-Seq	Thermo Fisher Scientific	Cat. #:A1083708
Commercial assay or kit	Direct-zol RNA Microprep Kit	Zymo Research	Cat. #:R2060
Commercial assay or kit	Illumina Stranded mRNA Prep, Ligation	Illumina	Cat. #:20040532
Commercial assay or kit	TruSeq Stranded Total RNA Library Prep Gold	Illumina	Cat. #:20020598
Commercial assay or kit	Vermiculite	VS Kakou
Commercial assay or kit	Supermix A	Sakata Seed Corp.	Cat. #:72000083
Commercial assay or kit	Cell strainer (100 µm pore size)	Corning	Cat. #:431752
Commercial assay or kit	Disposable hemacytometers	Funakoshi	Cat. #:521-10
Commercial assay or kit	Maxwell RSC Plant RNA Kit	Promega	Cat. #:AS1500
Commercial assay or kit	ReverTraAce qPCR RT Kit	TOYOBO	Cat. #:FSQ-101
Commercial assay or kit	THUNDERBIRD Next SYBR qPCR Mix	TOYOBO	Cat. #:QPX-201
Chemical compound, drug	RocA	Sigma-Aldrich	Cat. #:SML0656
Chemical compound, drug	Aglafoline	MedChemExpress	Cat. #:HY-19354
Chemical compound, drug	ADP	Fujifilm Wako Chemicals	Cat. #:019-25091
Chemical compound, drug	AMP-PNP	Roche	Cat. #:10102547001
Chemical compound, drug	G418	Fujifilm Wako Chemicals	Cat. #:078-05961
Software, algorithm	Trinity	https://github.com/Trinotate/Trinotate/wiki		Grabherr et al., 2011
Software, algorithm	Trinotate	https://github.com/Trinotate/Trinotate/wiki		Haas et al., 2013
Software, algorithm	MUSCLE	https://www.ebi.ac.uk/Tools/msa/muscle/
Software, algorithm	ESPript 3.0	http://espript.ibcp.fr/ESPript/ESPript/		Robert and Gouet, 2014
Software, algorithm	BLASTp	https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/		Camacho et al., 2009
Software, algorithm	BLASTn	https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/		Camacho et al., 2009
Software, algorithm	MAFFT v7.480	https://mafft.cbrc.jp/alignment/software/		Katoh and Standley, 2013
Software, algorithm	trimAl v1.4.rev15	https://anaconda.org/bioconda/trimal/files		Capella-Gutiérrez et al., 2009
Software, algorithm	catfasta2phyml v1.1.0	https://github.com/nylander/catfasta2phyml
Software, algorithm	ModelTest-NG v0.1.6	https://github.com/ddarriba/modeltes		Darriba et al., 2020
Software, algorithm	RAxML-NG v0.9.0	https://github.com/amkozlov/raxml-ng		Kozlov et al., 2019
Software, algorithm	iTOL v6	https://itol.embl.de/		Letunic and Bork, 2021
Software, algorithm	Igor Pro v8.01	WaveMetrics: https://www.wavemetrics.com/products/igorpro
Software, algorithm	minimap2 v2.17-r941	https://anaconda.org/bioconda/minimap2/files		Li, 2018
Software, algorithm	flye v2.8.1-b1676	https://anaconda.org/bioconda/flye/files?page=2		Kolmogorov et al., 2019
Software, algorithm	nucmer	https://mummer4.github.io/manual/manual.html		Delcher et al., 2003
Software, algorithm	Fastp v0.21.0	https://github.com/OpenGene/fastp		Chen et al., 2018
Software, algorithm	RNAmmer	http://www.cbs.dtu.dk/services/RNAmmer/		Lagesen et al., 2007
Software, algorithm	tRNA-scan SE	http://lowelab.ucsc.edu/tRNAscan-SE/		Chan et al., 2021
Software, algorithm	STAR v2.7.0a	https://github.com/alexdobin/STAR		Dobin et al., 2013
Software, algorithm	DESeq2	https://bioconductor.org/packages/release/bioc/html/DESeq2.html		Love et al., 2014
Software, algorithm	DAVID	https://david.ncifcrf.gov/home.jsp		Huang et al., 2009a; Huang et al., 2009b
Software, algorithm	StringTie v2.2.1	https://github.com/gpertea/stringtie		Kovaka et al., 2019
Other	NGC chromatography system	Bio-Rad		High-performance liquid chromatography
Other	Infinite F-200 PRO	Tecan		Plate reader
Other	EnVision 2104 plate reader	PerkinElmer		Plate reader
Other	GloMax Navigator System	Promega	Cat. #: GM2010	Microplate luminometer
Other	Swinging-bucket rotor	Hitachi	Cat. #:T4SS31	Centrifuge rotor
Other	Centrifuge	Hitachi	Cat. #:CF16RXII	Centrifuge
Other	Multi-beads Shocker	YASUI KIKAI	Cat. #:MB2200(S)	Bead mill homogenizer
Other	Biotron	NK Systems	Cat. #:LPH-410S and NH350S	Biotron
Other	Glass spray	Sansho	Cat. #:81-1192	Spray
Other	Air compressor	NRK Japan	Cat. #:UP-5F	Air compressor
Other	6 mm trepan	Kai Medical	Cat. #:BP-60F	Biopsy Punch
Other	2 ml steel top tube	BMS	Cat. #:MT020-01HS	Sample tube
Other	Φ5-mm zirconia beads	Nikkato	Cat. #:5-4060-13	Zirconia beads
Other	Shakemaster NEO	BMS	Cat. #:BMS-mini16	Bead mill homogenizer
Other	Maxwell RSC 48 Instrument	Promega	Cat. #:AS8500	Automated nucleic acid purification platform
Other	MX3000P Real-Time qPCR System	Stratagene	Cat. #:401511	Real-time qPCR system