Table 1. Summary of Kd (µM) between eIF4A protein and RNAs.
A fluorescence polarization assay between FAM-labeled RNA ([AG]10) and the indicated recombinant proteins was conducted to measure Kd in the presence of DMSO, RocA, or aglafoline. ND, not determined.
| [AG]10 | |||||
|---|---|---|---|---|---|
| ADP + Pi | AMP-PNP | ||||
| Protein | DMSO | RocA | Aglafoline | DMSO | RocA |
| H. sapiens WT Phe163 | 0.42 ± 0.061 | 11 ± 2.9 | 0.067 ± 0.023 | ||
| H. sapiens Phe163His | 4.0 ± 0.71 | 16 ± 2.7 | 0.11 ± 0.025 | ||
| H. sapiens Phe163Gly | 14 ± 2.0 | 21 ± 6.7 | 0.58 ± 0.13 | ||
| O.sinensis WT His154 | 0.11 ± 0.022 | 41 ± 11 | 0.090 ± 0.014 | ||
| O.sinensis His154Gly | 0.85 ± 0.12 | 27 ± 7.0 | 0.37 ± 0.046 | ||
| Ophiocordyceps sp. BRM1 Gly172Phe | ND | 0.27 ± 0.050 | 0.11 ± 0.021 | 110 ± 58 | 0.053 ± 0.023 |
| Ophiocordyceps sp. BRM1 Gly172His | ND | 3.9 ± 0.98 | 1.5 ± 0.14 | 3.3 ± 0.83 | 0.051 ± 0.0091 |
| Ophiocordyceps sp. BRM1 WT Gly172 | ND | 17±7.4 | 2.6±0.40 | 7.1±2.3 | 0.23±0.050 |